特(Te)别(Bie)提(Ti)示(Shi)：(?)本(Ben)公(Gong)司(Si)的(De)所(Suo)有(You)产(Chan)品(Pin)仅(Jin)可(Ke)用(Yong)于(Yu)科(Ke)研(Yan)实(Shi)验(Yan)，(?)严(Yan)禁(Jin)用(Yong)于(Yu)临(Lin)床(Chuang)医(Yi)疗(Liao)及(Ji)其(Qi)他(Ta)非(Fei)科(Ke)研(Yan)用(Yong)途(Tu)！(?)

商(Shang)品(Pin)介(Jie)绍(Shao)：(?)
别(Bie)    名(Ming)：(?)LAP18; C1orf215; Lag; LAP 18; LAP18; LAP-18; Leukemia associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP 18; OP18; p18; p19; Phosphoprotein 19; Phosphoprotein p19; PP17; PP19; PR22; Pr22 protein; Prosolin; Protein Pr22; SMN; Stathmin; Stathmin

抗(Kang)体(Ti)来(Lai)源(Yuan)：(?)Mouse

克(Ke)隆(Long)类(Lei)型(Xing)：(?)Monoclonal

克(Ke) 隆(Long) 号(Hao)：(?)14F6

交(Jiao)叉(Cha)反(Fan)应(Ying)：(?)Human

产(Chan)品(Pin)应(Ying)用(Yong)：(?)IHC-P=1:200-500,IHC-F=1:200-500,IF=1:200-500

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理(Li)论(Lun)分(Fen)子(Zi)量(Liang)：(?)17kDa

性(Xing)    状(Zhuang)：(?)Liquid

浓(Nong)    度(Du)1mg/ml

免(Mian) 疫(Yi) 原(Yuan)：(?)KLH conjugated synthetic peptide derived from human Stathmin 1

亚(Ya)    型(Xing)：(?)IgG

纯(Chun)化(Hua)方(Fang)法(Fa)：(?)affinity purified by Protein A

缓(Huan) 冲(Chong) 液(Ye)：(?)0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保(Bao)存(Cun)条(Tiao)件(Jian)：(?)Shipped at 4℃(?). Store at -20 °(?)C for one year. Avoid repeated freeze/thaw cycles.

注(Zhu)意(Yi)事(Shi)项(Xiang)：(?)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产(Chan)品(Pin)介(Jie)绍(Shao)：(?)This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. 
抗(Kang)体(Ti)的(De)多(Duo)样(Yang)性(Xing)：(?)
抗(Kang)体(Ti)的(De)异(Yi)质(Zhi)性(Xing)。(?)抗(Kang)体(Ti)的(De)组(Zu)成(Cheng)极(Ji)为(Wei)复(Fu)杂(Za)，(?)是(Shi)由(You)成(Cheng)千(Qian)上(Shang)万(Wan)、(?)多(Duo)种(Zhong)多(Duo)样(Yang)的(De)免(Mian)疫(Yi)球(Qiu)蛋(Dan)白(Bai)（(?)Ig）(?)分(Fen)子(Zi)所(Suo)组(Zu)成(Cheng)。(?)这(Zhe)些(Xie)Ig分(Fen)子(Zi)在(Zai)形(Xing)状(Zhuang)、(?)大(Da)小(Xiao)、(?)结(Jie)构(Gou)以(Yi)及(Ji)氨(An)基(Ji)酸(Suan)的(De)组(Zu)成(Cheng)和(He)排(Pai)列(Lie)上(Shang)，(?)既(Ji)相(Xiang)似(Si)，(?)又(You)有(You)差(Cha)别(Bie)。(?)由(You)于(Yu)有(You)差(Cha)别(Bie)，(?)它(Ta)们(Men)的(De)电(Dian)泳(Yong)活(Huo)性(Xing)就(Jiu)有(You)很(Hen)大(Da)的(De)变(Bian)化(Hua)。(?)

因(Yin)为(Wei)抗(Kang)体(Ti)具(Ju)有(You)与(Yu)抗(Kang)原(Yuan)决(Jue)定(Ding)簇(Cu)相(Xiang)对(Dui)应(Ying)的(De)结(Jie)合(He)部(Bu)位(Wei)（(?)抗(Kang)原(Yuan)结(Jie)合(He)簇(Cu)）(?)，(?)所(Suo)以(Yi)抗(Kang)体(Ti)与(Yu)抗(Kang)原(Yuan)的(De)结(Jie)合(He)具(Ju)有(You)特(Te)异(Yi)性(Xing)。(?)另(Ling)一(Yi)方(Fang)面(Mian)，(?)抗(Kang)体(Ti)本(Ben)身(Shen)是(Shi)一(Yi)种(Zhong)蛋(Dan)白(Bai)质(Zhi)，(?)具(Ju)有(You)本(Ben)身(Shen)的(De)氨(An)基(Ji)酸(Suan)组(Zu)成(Cheng)、(?)排(Pai)列(Lie)和(He)立(Li)体(Ti)结(Jie)构(Gou)，(?)对(Dui)异(Yi)种(Zhong)动(Dong)物(Wu)来(Lai)说(Shuo)，(?)它(Ta)又(You)是(Shi)抗(Kang)原(Yuan)。(?)各(Ge)类(Lei)Ig都(Du)具(Ju)有(You)可(Ke)用(Yong)血(Xue)清(Qing)学(Xue)方(Fang)法(Fa)检(Jian)出(Chu)的(De)抗(Kang)原(Yuan)特(Te)异(Yi)性(Xing)，(?)它(Ta)们(Men)表(Biao)现(Xian)出(Chu)不(Bu)同(Tong)的(De)血(Xue)清(Qing)学(Xue)类(Lei)型(Xing)。(?)

公(Gong)司(Si)正(Zheng)在(Zai)售(Shou)的(De)产(Chan)品(Pin)：(?)

免(Mian)疫(Yi)荧(Ying)光(Guang)技(Ji)术(Shu)的(De)实(Shi)验(Yan)步(Bu)骤(Zhou)：(?)

一(Yi)、(?)准(Zhun)备(Bei)好(Hao)试(Shi)剂(Ji)与(Yu)仪(Yi)器(Qi)：(?)

磷(Lin)酸(Suan)盐(Yan)缓(Huan)冲(Chong)盐(Yan)水(Shui)、(?)荧(Ying)光(Guang)标(Biao)记(Ji)的(De)抗(Kang)体(Ti)溶(Rong)液(Ye)、(?)缓(Huan)冲(Chong)甘(Gan)油(You)、(?)搪(Tang)瓷(Ci)桶(Tong)三(San)只(Zhi)、(?)有(You)盖(Gai)搪(Tang)瓷(Ci)盒(He)一(Yi)只(Zhi)、(?)荧(Ying)光(Guang)显(Xian)微(Wei)镜(Jing)、(?)玻(Bo)片(Pian)架(Jia)、(?)滤(Lu)纸(Zhi)、(?)37℃(?)温(Wen)箱(Xiang)等(Deng)。(?)

二(?)、(?)实(Shi)验(Yan)步(Bu)骤(Zhou)

1.滴(Di)加(Jia)0.01mol/L，(?)pH7.4的(De)PBS于(Yu)待(Dai)检(Jian)标(Biao)本(Ben)片(Pian)上(Shang)，(?)十(Shi)分(Fen)钟(Zhong)后(Hou)弃(Qi)去(Qu)，(?)使(Shi)标(Biao)本(Ben)保(Bao)持(Chi)一(Yi)定(Ding)湿(Shi)度(Du)。(?)

2.滴(Di)加(Jia)适(Shi)当(Dang)稀(Xi)释(Shi)的(De)荧(Ying)光(Guang)标(Biao)记(Ji)的(De)抗(Kang)体(Ti)溶(Rong)液(Ye)，(?)使(Shi)其(Qi)覆(Fu)盖(Gai)标(Biao)本(Ben)，(?)置(Zhi)于(Yu)有(You)盖(Gai)搪(Tang)瓷(Ci)盒(He)内(Nei)，(?)保(Bao)温(Wen)一(Yi)定(Ding)时(Shi)间(Jian)以(Yi)三(San)十(Shi)分(Fen)钟(Zhong)为(Wei)参(Can)考(Kao)。(?)

3.取(Qu)出(Chu)玻(Bo)片(Pian)，(?)置(Zhi)玻(Bo)片(Pian)架(Jia)上(Shang)，(?)先(Xian)用(Yong)0.01mol/L，(?)pH7.4的(De)PBS冲(Chong)洗(Xi)后(Hou)，(?)再(Zai)按(An)顺(Shun)序(Xu)过(Guo)0.01mol/L，(?)pH7.4的(De)PBS三(San)缸(Gang)浸(Jin)泡(Pao)，(?)每(Mei)缸(Gang)三(San)到(Dao)五(Wu)分(Fen)钟(Zhong)，(?)并(Bing)不(Bu)停(Ting)地(Di)摇(Yao)晃(Huang)振(Zhen)荡(Dang)。(?)

4.取(Qu)出(Chu)玻(Bo)片(Pian)，(?)用(Yong)滤(Lu)纸(Zhi)吸(Xi)去(Qu)多(Duo)余(Yu)水(Shui)分(Fen)，(?)但(Dan)不(Bu)使(Shi)标(Biao)本(Ben)干(Gan)燥(Zao)，(?)加(Jia)一(Yi)滴(Di)缓(Huan)冲(Chong)甘(Gan)油(You)，(?)以(Yi)盖(Gai)玻(Bo)片(Pian)覆(Fu)盖(Gai)。(?)

5.立(Li)即(Ji)用(Yong)荧(Ying)光(Guang)显(Xian)微(Wei)镜(Jing)观(Guan)察(Cha)。(?)观(Guan)察(Cha)标(Biao)本(Ben)的(De)特(Te)异(Yi)性(Xing)荧(Ying)光(Guang)强(Qiang)度(Du)。(?)